By using this techniques, the catheter complications, in particular obstruction, bowel and vaginal cuff perforation were greatly reduced61. Saroglitazar suppresses the hepatocellular carcinoma induced by Upon intravenous and intraperitoneal injection of stressed MSA, fluorescent "hotspots" were observed in the spleens, livers and lungs. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane Since that time, the literatures using stem cells for IBD have expanded. Epub 2020 Mar 18. At the end of laparoscopic . In this study, the therapeutic efficiency of three different MSCs delivery routes (IP, AI and IV) were compared on DSS-induced colitis mouse model. By submitting a comment you agree to abide by our Terms and Community Guidelines. PubMed Central [8], A good example of how intraperitoneal injections work is depicted through "The distribution of salicylate in mouse tissues after intraperitoneal injection" because it includes information on how a drug can travel to the blood, liver, brain, kidney, heart, spleen, diaphragm, and skeletal muscle once it has been injected intraperitoneally.[9]. TSG-6 might be a major anti-inflammatory mechanism of MSCs, since not only in colitis model, the therapeutic effect of TSG-6 remotely secreted by MSCs were also identified in many other disease model46,48,49,50. Alavi S, Haeri A, Mahlooji I, Dadashzadeh S. Pharm Res. This study was designed (1) to examine a novel route of opioid administration, the intraperitoneal injection; (2) to compare this to interpleural application, and (3) to compare opioid with local anesthetic effects under both conditions.Methods. Pharm Res. [4] In 1964, a study delivered chemical agents such as acetic acid, bradykinin, and kaolin to mice intraperitoneally in order to study a "squirming" response. supply of IGF-I to adolescent and adult animals or humans is not known. 2A), which guarantee the less interference from the background signal that coming from free dye. IP injected human MSC rapidly formed aggregates with mouse macrophages and B220+ lymphocytes and attached to the walls of the peritoneal cavity. The natural history of adult Crohns disease in population-based cohorts. found MSCs improved cardiac function by a remote way46. Intraperitoneal Drug Delivery for Ovarian Cancer: Why, How, Who, What It is more often applied to animals than to humans. Stem cells (Dayton, Ohio) 26, 279289, doi: 10.1634/stemcells.2007-0454 (2008). Schrepfer, S. et al. Intraperitoneal Injection of Oncolytic Viruses H101 for Patients With Refractory Malignant Ascites. Course of Crohns disease after allogeneic marrow transplantation. Scientific Reports (Sci Rep) 6B). While this route is an easy to master, quick, suitable for chronic treatments and with low impact of stress on laboratory rodents, there is a common concern that it may not be an acceptable route for drug administration in experimental studies. In this study, the intraperitoneal injection was demonstrated as the superior way for MSCs administration in the setting of colitis amelioration. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Intratumoral injection of hydrogel-embedded nanoparticles enhances Total RNA was extracted with the RNAeasy Plus kit (QIAGEN). PDF Guidelines for the Research Use of Adjuvants - National Institutes of . Collagen deposition limits immune reconstitution in the gut. There are few examples of the use of intraperitoneal injections in humans cited in literature because it is mainly used to study the effects of drugs in mice. Dig Dis 28, 386394, doi: 10.1159/000320392 (2010). The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. After 5 days of DSS contained water, all the subject mice showed strong positive test of fecal occult blood. GFP+ cells were detected at the inflamed colon. Google Scholar. 17 The influence of the sex of the animal on anesthetic agents remains poorly researched and understood. Adhesions are a leading cause of secondary infertility in women, accounting for 15% to 20% of cases. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). ", "Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Intraperitoneal injection is widely used in rodents as a route of drug administration. Establishing a xenograft mouse model of peritoneal dissemination of Essentials of Human Nutrition - Jun 13 2022 . Mesenchymal Stem Cells Reduce Colitis in Mice via Release of TSG6, Independently of Their Localization to the Intestine. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Gut 63, 588597, doi: 10.1136/gutjnl-2013-304636 (2014). DiR-labeled cells were injected into DSS mice by three different ways. These early uses of Intraperitoneal injections provide good examples of how the delivery method can be used, and provides a base for future studies on how to properly inject mice for research. A Simple Mouse Model for the Study of Human Immunodeficiency Virus Supplies: 2020 Apr;17(4):511-522. doi: 10.1080/17425247.2020.1736551. Intraperitoneal injection (IP), Intravenous injection (IV) or anal Caplan, A. I. (B) Quantification of FoxP3+ cells, the IP>IA and IV>DSS control, n=6 mice/group; n=3 for the control group. eCollection 2021. 2B). Article In another Phase I clinical trial, patients with ovarian cancer were injected intraperitoneally with dl1520 in order to study the effects of a replication-competent/-selective virus. Google Scholar. Surg Clin North Am 87, 727741, doi: 10.1016/j.suc.2007.03.004 (2007). Am J Gastroenterol 105, 289297, doi: 10.1038/ajg.2009.579 (2010). Though genetic and environmental factors are believed to be involved in the disease, not much progress has been made on therapy. Stem cells and development 22, 181192, doi: 10.1089/scd.2012.0373 (2013). It is estimated that as many as 1.4million Americans and 2.4million Europeans are suffering from these diseases1,2. (A) Scheme of the treatment, the day of MSCs injection was defined as day 0. However, doubts exist over the use of Fe 3 O 4-nanoparticles in human beings. Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. and H.H. Cardiovascular research 96, 208209; discussion 210203, doi: 10.1093/cvr/cvs202 (2012). The histological score was defined as the sum of the two parameters (total score=E+I). DSS only served as control. The site is secure. Bookshelf This work is licensed under a Creative Commons Attribution 4.0 International License. Near-infrared imaging and quantification of MSCs transplants. Statistical significance was determined using one-way ANOVAs or Students t tests. In anal injection group, same amount of cells were suspended in 200L diluted Matrigel in PBS (1:20) and the cell suspension was instilled into colonic lumen by a syringe with flexible catheter 4cm in length and 2mm in diameter, according to the previous study64. injection, or EBI) remains the most humane method available. The delivery of OK-432 occurred a total of four times in a span of one week. 3; Supplementary Figure S6). Injected dissociative agent combinations. Nature medicine 18, 618623, doi: 10.1038/nm.2695 (2012). Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? Changing Crohns disease management: need for new goals and indices to prevent disability and improve quality of life. Easier to inject that intravenous. Sci Rep 6, 30696 (2016). Unable to load your collection due to an error, Unable to load your delegates due to an error. We notice that the mean density of collagen was lowest in IP group, however, IP group did not exhibit less collagen deposition than the AI group, probably due to the scatter points (Fig. Intraperitoneal Route of Drug Administration: Should it Be Used in Inflammatory Scores were calculated using a score system66: Epithelium (E): 0, normal morphology; 1, loss of goblet cells; 2, loss of goblet cells in large areas; 3, loss of crypts; 4, loss of crypts in large areas. MPO activity per gram of wet tissue was calculated as: MPO activity (U/g wet tissue)=(A450) (13.5)/tissue weight (g). adult mice at 8-10 weeks of age were first anaesthetized by intraperitoneal injection of 20 mg/ml Avertin (Sigma-Aldrich) (15 l/g . Inflammatory bowel disease (IBD). Comparative analysis of paracrine factor expression in human adult mesenchymal stem cells derived from bone marrow, adipose and dermal tissue. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. Parenteral administration routes include, but are not limited to epidermal, intraarterial, intramuscular (IM and depot IM) , intraperitoneal (IP) , intravenous (IV) , intrastemal injection or infusion techniques, intranasal (inhalation) , intrathecal, injection into the stomach, subcutaneous injections (subcutaneous (SQ and depot SQ . Slider with three articles shown per slide. One article reviews the injection of sodium pentobarbital to euthanize rodents intraperitoneally. ADS These immune cells may interact with MSCs and exert regulatory functions to MSCs. Intraperitoneal Injection - an overview | ScienceDirect Topics These time-tested therapies may perform well on some patients, but also exhibit inadequacies in efficacy. One study utilized IP injections to study pain in the abdomen after a hysterectomy when administering anesthetic continuously vs patient-controlled. In a case of a ruptured hepatocellular carcinoma, it was reported that the patient was treated successfully through the use of an intraperitoneal injection of OK-432, which is an immunomodulatory agent. A . We have proposed that safe and effective protection against the development of adult onset cancers may be achieved by vaccination against tissue-specific self-proteins that are "retired" from expression at immunogenic levels in normal tissues as we age, but are overexpressed in emerging tumors. The GFP+ MSCs were found at inflamed colon in both IP and AI groups 24h after MSCs injection. X.W., Y.H., Y.N. One example is using ultrasound-navigation for puncture. The first major problem with IV route is the pulmonary first-pass effects, which cause significant entrapment of cells15. Blood 122, e23e32, doi: 10.1182/blood-2013-04-495119 (2013). Colonic mRNA expression of inflammatory mediators such as TNF-, IL-6, IL-1, IL-10 and IFN- were analyzed by real time-PCR in order to evaluate the local inflammatory environment (Fig). For some severe cases, IBD may lead to the life-threaten complications5. In patients with peritoneal surface malignancies, perioperative IP chemotherapy has been used with good results. These findings may benefit clinic practice. Choi, H., Lee, R. H., Bazhanov, N., Oh, J. Y. Generally, MSCs treatments tend to decrease the pro-inflammatory cytokine (TNF-, IL-6, IL-1b and IFN-) and increase the anti-inflammatory cytokine (IL-10). Currently, therapy still largely relies on empirical and often implemented in a stepwise fashion: progressing through 5-aminosalicylate compounds, corticosteroids, immunomodulatory drugs and finally anti-TNF drugs7. However, whether the lymphocytes actually . Incidence and phenotype of inflammatory bowel disease based on results from the Asia-pacific Crohns and colitis epidemiology study. DiR labeling showed no harm to the MSCs viability13 (Supplementary Figure S3). There are increasing evidences showed that MSCs mediate their immunomodulatory effects through the induction of regulatory T cells (Treg cells)14. However, the therapeutic impacts of cell delivery route that is critical for clinical translation are currently poorly understood. It means IP injection is more feasible and reasonable for clinical settings. This allows researchers to observe the effects of a drug in a shorter period of time, and allows them to study the effects of drugs on multiple organs that are in the peritoneal cavity at once. After wishing with PBS, the sections were incubated 1h at room temperature with secondary antibody, then visualized by diaminobenzidine (DAB). Kushnir, C. L., Fleury, A. C., Silver, D. F. & Spirtos, N. M. Intraperitoneal Catheter Placement: The Hammock Technique. M.W., C.L. It also shows how easily substances are absorbed through the peritoneal cavity after an IP injection. Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6. Transplantation proceedings 39, 573576, doi: 10.1016/j.transproceed.2006.12.019 (2007). Intradermal. Factors such as peritoneal inflammation, surface area, peritoneal blood flow, time of contact, etc, influence the drug transfer. 2014 Apr 26;(4):CD005284. Zhu, H. et al. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway. Cells, tissues, organs 169, 1220 (2001). Yu-Lung Chang, Huei-Yu Lo, Chin-Kan Chan, Xing-hua Pan, Qing-qing Li, Guang-ping Ruan, Hongsheng Yang, Rui Feng, Shenghong Zhang, H. R. Wardill, C. E. M. de Mooij, N. M. A. Blijlevens, Suze A. Jansen, Edward E. S. Nieuwenhuis, Caroline A. Lindemans, Scientific Reports The problem is obvious, the solution is not: numbers do matter in cardiac cell therapy! Levy, O. et al. Intraperitoneal Versus Interpleural Morphine or Bupivacaine for Pain In their study, PKH26 (a yellow-orange fluorescent dye) labeled PCs were injected intraperitoneally into healthy rat. However, they found the migrated PCs was very few and these may attribute to: 1) the recipient are heathy rat. Thank you for visiting nature.com. All the tissue sections in this study were scan and analyzed with Virtual slide scanning system (VS120-S, Olympus). anesthesia). Intraperitoneal injection - Wikipedia At 72h, the IP groups showed significant higher level of TSG-6 than AI groups and DSS control (P=0.023 and P=0.005, respectively). IP, intraperitoneal injection; IV, intravenous injection; AI anal injection; n = 6-8 for treatment group, n = 3 for DSS control. Yui, S. et al. Acceptable Method (s) Injected barbiturates and barbiturate combinations. Google Scholar. Intraperitoneal injections may also allow too-rapid entry of bacteria or LPS into the blood. Dou, S., Smith, M., Wang, Y., Rusckowski, M. & Liu, G. Intraperitoneal injection is not always a suitable alternative to intravenous injection for radiotherapy. But how these miracle cells reach to the lumen and by which possible migration route? Ex-vivo study was carried out at 1 day post-injection. Berberich, S., Forster, R. & Pabst, O. Gastroenterology 132, 944954, doi: 10.1053/j.gastro.2006.12.029 (2007). Stem cells (Dayton, Ohio) 24, 386398, doi: 10.1634/stemcells.2005-0008 (2006). seem to have found the key drug of MSCs in the treatment of colitis, TSG-647. It was concluded that there is evidence that IP may not be the best method of euthenasia due to possibilities of missinjection. Interferon-gamma (IFN-gamma)- and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. Histological evaluation was completed in a double-blind fashion by two independent pathologist. Bull Emerg Trauma. In the meanwhile, the colonic homogenates were collected and colonic IL-10, TNF- were measured by ELISA assays. Haematologica 93, 14571465, doi: 10.3324/haematol.12553 (2008). In contrast, GFP+ cells were showed in the lumen for AI group and lamina propria for IV group (Fig. The increasing evident indicated that cell-cell contact between MSCs and immune cells may also be of importance52,53,54. We also evaluated serum TSG-6 in three MSCs administration route. In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. All fluorescent imaging procedures were conducted on small animal imaging system (IVIS Kinetics; Caliper Life Science). Collagen Deposition was assessed with phosphomolibidic acid-picrosirius red staining. Generally, the FoxP3 expression was elevated in MSCs treatment (Fig. IBD can be painful and persistent for life, which might decrease the quality of life. For intraperitoneal administration, restrain the mouse with one hand and tilt the head backward to expose the abdomen. Mice were made diabetic by intraperitoneal injection of streptozotocin, which led to a 2- to 2.5-fold increase in plasma glucose. Google Scholar. An example of how intraperitoneal injections can be optimized is depicted in a study where IP injections are used to deliver anesthesia to mice. This case is a good example of how IP injections can be used to deliver a drug that can help to treat or cure a medical diagnosis over the use of other routes of delivery. A similar increase in mouse cytokines in the peritoneum was seen after IP injections of human fibroblasts. Liang, J. et al. Kalchenko, V. et al. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. In IP group, cells were found accumulation at the epithelium; In AI group, cells were mainly in the lumen; In IV group, cells were fewer and located in lamina propria. A relatively short needle (e.g., 0.5-inch or less) is suggested to help prevent puncture of the intestines or cecum. In our study, we also identify MSCs existence in nearby MLN. 2A). However, no significant TSG-6 difference was found between AI and IV groups. The bilateral femurs and tibias were aseptically excised, stripped of connective tissues and then stored in PBS. prepared Figure 4. Sala, E. et al. By systemic evaluation of epithelium and lymphocyte infiltration, the IP injection group showed the relatively lower severity score compared with AI group (P=0.034) and IV (P=0.002), respectively. drug availability and human abuse potential; 10. compatibility with species, age, and health status; 11. Lu, Z., Wang, J., Wientjes, M. G. & Au, J. L. Intraperitoneal therapy for peritoneal cancer. Intraperitoneal injection of 0.5-14.6 mg/kg 17- or 37-nm gold to mice produced an increase of brain dopamine, . 1B). The IP 'option' was not adopted, however, because the standard treatment had shifted to IV cisplatin plus . Mouse insulin gradually decreased and was no more detectable 13 weeks after STZ injection. Proceedings of the National Academy of Sciences of the United States of America 99, 89328937, doi: 10.1073/pnas.132252399 (2002). Hepatic and renal . The article overviews whether IP is the best option for euthanization based on evidence associated with welfare implications. Srivastava, A. K. & Bulte, J. W. Seeing stem cells at work in vivo. [14] The symptoms of this included impairment of cognitive and psychomotor abilities. An official website of the United States government. The fate of human peripheral blood lymphocytes after - PubMed Select search scope, currently: articles+ all catalog, articles, website, & more in one search; catalog books, media & more in the Stanford Libraries' collections; articles+ journal articles & other e-resources In most experimental studies, the MSCs treatment usually require at least 1106 cells/mouse, more frequently, a dose as high as 5106 cells/mouse to observe any effect12. The MSCs may exert their therapeutic effects on mainly three following aspects: firstly, to differentiate and replace the damage tissue; secondly, to secrete soluble immunomodulatory bioactive molecules and establish a regenerative milieu; thirdly, cell-to-cell contact and interaction with immune cells. doi: 10.1002/14651858.CD005284.pub3. In 2018, a patient with stage IV ovarian cancer and peritoneal metastases was injected intraperitoneally with 12g of mixed cannabinoid before later being hospitalized. Ng, S. C. et al. [13] The patient was a 51-year-old male who was hospitalized. Epidemiology and natural history of inflammatory bowel diseases. From two streams of research, experimental and clinical, stem cell therapy showed promising results. Intraperitoneal (ml) Footpad (ml) Mouse <0.1 . The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. Subcutaneous Compared with Intraperitoneal Ketamine-Xylazine for Comparison of the Effects of Intraperitoneal Injection with Carbon The peritoneal micromilieu commits B cells to home to body cavities and the small intestine. Several medications such as antibiotics are given via the intraperitoneal (IP) route in PD patients to treat episodes of peritonitis. PDF EUTHANASIA REFERENCE MANUAL - HumanePro Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue. A., Ong, J. F. & Karp, J. M. Mesenchymal stem cells: immune evasive, not immune privileged. Reduced MPO activities were identified in all MSCs groups, of which IP injection showed lower MPO activity (P=0.001vs. IV and P=0.0158vs. AI; Fig. Would you like email updates of new search results? Blood and marrow stem cell transplants in autoimmune disease. In consistent with Scaldaferris work, the increased TSG-6 after MSCs transplantation was also identified. Journal of molecular neuroscience: MN 44, 178185, doi: 10.1007/s12031-010-9446-6 (2011). [17] Contrast agents were administered intraperitoneally instead of intravenously to avoid errors and challenges. . To compare the Ki-67 expression (one of the markers of cell proliferation) among the groups, the colon tissues were collected on day 7 (Fig. Goncalves Fda, C. et al. Chemotactic cytokines might be involved and responsible for MSCs attraction. Pentobarbital sodium Injection for Animal Use - Drugs.com MathSciNet 6A). But this study at least pointed out the possible migration route for cells by intraperitoneal injection. Ahmadian Kia, N. et al. evaluated the therapeutic efficacy of different MSCs sources and delivery routes in experimental emphysema37. As mentioned above, a commonly used cell dose is 1106 cells/30g mouse, which would be equivalent to 33106/kg or approximately 2.3billion cells for a 70kg adult. PDF An improved mouse model of sepsis based on intraperitoneal injections Evid Based Complement Alternat Med. MSCs have been well characterized regarding their ability to produce a range of cell cytokines, which have a profound effect on modulating the immune system. The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. & Ramesh, T. Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis. Twenty-five Kunming mice were exposed to Fe 3 O 4-nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. and JavaScript.
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